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Oligonucleotide DNA microarrays were produced targeting the genes of enterococcal glycopeptide resistance gene clusters.
Microarrays were produced by Agilent by in situ synthesis technology.
cDNA microarrays were produced by the Stanford Functional Genomic Facility and contained 43,197 human probes representing 26,524 Unigene cluster IDs (12,466 ENSEMBL annotated genes) spotted on Corning Ultra GAPS slides.
HEEBO oligonucleotide microarrays were produced by Stanford Functional Genomic Facility.
miRNA microarrays were produced as previously described [ 26, 27].
The microarrays were produced by Agilent © and are available under the array number 024664.
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Secondly, since the protein microarrays are produced with recombinant human proteins expressed in Sf9 insect cells a proportion of these substrates could be misfolded, possibly precluding their modification or promoting their artificial modification.
Most currently used genomic copy-number profiling microarrays are produced in academic settings, and the resolution of these microarrays varies depending on the type and number of genomic targets selected, the protocols used, and the data-analysis tools employed.
Glass DNA microarrays are produced by the robotic application of DNA to a glass surface; cDNAs or genomic clones are usually applied [ 6, 7], although oligonucleotides may also be used [ 13].
A total of 252 sequence fragments corresponding to full length CTV genomes and genomic fragments tiled on the microarray were produced by the resequencing analysis.
The R values of the linear regions are very close to -1, with slopes between -0.842 and -0.935, indicating that both the qPCR and the microarrays are producing sample-responsive and internally consistent measurements of miR-296 and miR-494 at these concentration levels.
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