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Antigen microarrays were prepared and studied as described previously [1], [2], [3].
The Affymetrix microarrays were prepared and hybridized using standard Affymetrix protocols [9], [36].
The microarrays were prepared such that each array was composed of two slides of 44,000 spots each.
DNA microarrays were prepared on glass microscope slides according to a photolabile deprotection strategy that has been previously described [6].
The microarrays were prepared for hybridization by first binding the DNA to the slides by rehydrating the microarray over steam, drying on a heat block (∼70°C), then placing in a UV stratalinker at 300 mJ.
Tissue microarrays were prepared.
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Larval RNA for microarrays was prepared from 6 sets of 50 mid 3rd instar larvae reared in microgravity and collected on the day of landing of the space shuttle, and the same number of control larvae raised on ground.
Material used for the microarrays was prepared and handled as described in [ 76].
The RNA material used to interrogate the microarrays was prepared separately.
RNA target for microarrays was prepared using the 3′ IVT Express Kit (Affymetrix, Santa Clara, CA) according to manufacturer's instructions starting with 200 ng of total RNA.
RNAs to be used for microarray were prepared as previously described [18].
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microarrays were provided
microarrays were analyzed
microarrays were blocked
microarrays were performed
microarrays were employed
microarrays were collected
microarrays were hybridized
microarrays were used
microarrays were constructed
microarrays were incubated
microarrays were controlled
microarrays was prepared
microarrays were included
microarrays were excluded
microarrays were synthesized
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