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Working at a microarray core facility is not where many of us thought we would be today, simply because microarrays were not as popular (or even around!) when we graduated. .
These findings indicate that the differences in regulation by drugs in the microarrays were not due to the selection of the 6 hour time-point.
The branches of pathways containing genes that are not expressed on microarrays were not considered functional.
When this study was started, cDNA microarrays were not used yet as a standard of classification for clinical practice and design of therapeutic strategy.
Due to lack of probe replication, 27 features on the microarrays were not included in analyses, resulting in 11,604 traits for further analysis.
The distributions of the expected values in the five mouse datasets were almost the same (Table 3), suggesting that differences in microarrays were not significant.
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Standard microarrays are not conductive which results in charge buildup during SIMS analysis.
However, though we detected many alternative splicing events using the whole exon chip, we are aware that these microarrays are not be able to detect exhaustively all alternative splicing in a given tissue.
This issue is difficult to address experimentally because the regulatory elements in the genes that are differentially regulated by E2 and ERβ-selective agonists as observed with the microarrays are not known.
Also rabbit microarrays are not available.
Nevertheless available microarrays are not completely reliable for this purpose.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com