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First generation MHC microarrays were generated by direct spotting of the pMHC of interest onto the microarray slide.
The GNF protein microarrays were generated by using 2354 human recombinant proteins from the GNF library, which were produced as described previously7.
DAT image and CEL intensity files of the microarrays were generated using the Affymetrix GeneChip® Command Console (version 0.0.0.676, Affymetrix).
Since microarrays were generated using Col-0 probes, it is difficult to say whether these genes were specifically expressed in diseased plants in this particular accession, or are widely sequence divergent in Nd-1 plants.
Human prostate tissue samples from the paraffin embedded whole mount blocks from the 43 patients originally characterized for BPH pathology severity and utilized for corresponding TZ derived RNA were obtained from the Vanderbilt Tissue Acquisition Core via the Department of Pathology and tissue microarrays were generated by one of the authors (MPR).
Microarrays were generated and probes hybridized as described [ 15].
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We describe a novel, simple and low-cost protein microarray strategy wherein the microarrays are generated by printing expression ready plasmid DNAs onto slides that can be converted into protein arrays on-demand.
Report files summarising the quality of target and control detection for each microarray were generated by GeneChip Operating Software Version 1.4 (GCOS) using the MAS5.0 algorithm (Affymetrix, Santa Clara, CA, USA).
All cDNA clones spotted on the microarray were generated by PCR using GF200 primer pairs, therefore all clones contains 110 bps vector sequence on their GF200 forward primer side.
Recently, new technologies such as improved genome sequencing and microarrays are generating huge quantities of genomic information.
The Staphylococcal protein microarray was generated by spotting purified recombinant proteins (0.5 mg/ml in a final glycerol concentration of 40%) in 16 replicates on nitrocellulose coated slides (FAST slides; Whatman) using the ink-jet spotter Marathon (Arrayjet) resulting in spots of ~110 μm in diameter.
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