Sentence examples for microarrays were done from inspiring English sources
cDNA microarrays were done to determine and compare the native expression levels of various cell cycle proteins expressed in G0-G1, S, G2-M phases and other upstream molecules, in both glioma and their treatment with hUCBSC.
First strategy, the same analyses with 48 microarrays were done again twice: one not using the total number of genes (i.e. 22 283 gene probesets) but only the 25% of the genes that showed the largest variance; and another using only the 25% of the genes that showed the highest signal.
Reverse transcription into cDNA, labeling of cDNA, hybridization and scanning of the microarrays were done according to the manufacturers' protocols.
The genes from each publication are listed in Supplementary file 1. RNA-extraction and hybridization to microarrays were done as previously described [ 23].
Microarrays were done in two (single mutants) or four (double mutants) replicate experiments, including both dye-swap technical replicates or/and biological replicates.
RNA extractions, amplification and labelling of RNA preparations, microarray hybridizations, as well as washing and scanning of microarrays were done as previously described [ 12, 13].
Intra-array normalization of raw signals from the 48 microarrays was done using Feature Extraction software 9.1.3.1 (Agilent).
The development of DNA microarray assays is hampered by two important aspects: processing of the microarrays is done under a single stringency condition, and characteristics such as melting temperature are difficult to predict for immobilized probes.
The construction of the tissue microarrays was done using a tissue arrayer (Beecher Instruments, Inc., Sun Prairie, WI, USA) [ 48].
Generation of hybridization signals of the microarrays was done using Microarray Suite 5.0 (MAS 5.0) (Affymetrix, CA) as previously described [ 21, 29– 329.
Reports about gene coverage in annotated microarrays are done by a series of CGI scripts providing an intuitive web interface to the database.
