Sentence examples for microarrays were determined from inspiring English sources
Gene expression log ratios (M = log2 treated/control) across the three microarrays were determined as described in Methods and plotted against log mean intensity values (A = 1/2log2 (treated * control) as shown in Figure 2[ 42[.
Gene expression profiles of cells surviving 0 (three microarrays), 1.0 μg/ml amp (four microarrays), 2.5 μg/ml amp (five microarrays), 5.0 μg/ml amp (four microarrays), 10.0 μg/ml amp (two microarrays), 20.0 μg/ml amp (one microarray) and 30.0 μg/ml amp (four microarrays) were determined.
The relationship between results obtained with qPCR-array and microarrays was determined.
The log2 ratio of RNA-Seq tumor samples to RNA-Seq human Common Reference Sample (which was the same RNA used for the 2-color microarrays) was determined.
Fluorescence of hybridized DNA microarrays was determined at 532 nm (Cy3) and 635 nm (Cy5) at 5 μm resolution with a GenePix 4000B laser scanner and GenePix Pro 6.0 software (Molecular Devices, Sunnyvale, CA, USA).
The specificity of the rabbit anti-ChREBP used for the immunohistochemical analysis of the tissue microarrays was determined by western analysis of a ChREBP knockdown cell line generated from MCF-7 cells.
The detection limits of the microarray were determined under these conditions by titration of chromosomal DNA and unlabelled amplicons followed by hybridisation to determine the levels of sensitivity that could be obtained with the microarray.
In addition, both the specificity and reproducibility of the microarray were determined using DNA from 5 healthy individuals.
Over-represented gene ontology categories for proteins changed by smoking and for proteins that were not detectably expressed by microarray were determined by comparing the corresponding RefSeq identifications numbers for these proteins against the complete set of 859 proteins detected by mass spectrometry in this set of experiments.
The correlation co-efficients between the set of signal ratios from each microarray were determined in a pair-wise manner.
Overall, mRNA expression levels of 17,816 RefSeq transcripts and full-length mRNA transcripts using the core probes of the exon microarray were determined, and the number of unique genes detected above background (DABG) was 11,112.
