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Although fold change in gene expression measured by real-time PCR was generally higher, there was a good correlation between the two data sets, and no genes that were deemed up-regulated with the microarrays were demonstrated to be down-regulated by qRT-PCR, and vice-versa (Table 2).
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The first demonstration of wide-field FLIM to read microarrays was demonstrated using time-gated imaging to improve the sensitivity for DNA profiling.[ 24] More recently, an automated instrument for unsupervised FLIM of multiwell-plate sample arrays was reported[ 25] that exploited the frequency domain (FD) fluorescence lifetime determination of FRET in a wide-field (non-sectioned) microscope.
The efficiency of developed microarrays was demonstrated using mouse IgG and goat anti-mouse IgG as a model affinity pair.
The potential for development of label-free, low-density DNA microarrays is demonstrated and is being pursued.
The adaption of data management and related tools to assemble the data, to check its quality, and to perform the low and high level analysis for a very large set of microarrays is demonstrated.
The quality of the printed microarray was demonstrated by co-hybridization with fluorescently labeled cDNA probes obtained from exponentially growing C. jeikeium cultures.
The performance of the optimized microarray was demonstrated in a pilot study.
Thus, the sensitivity, specificity, and discriminatory power of the microarray analysis were demonstrated to be satisfactory for foodborne pathogen identification and source attribution.
In another elegant work, ExMVs were demonstrated by microarray analysis to contain mRNAs for 1300 genes, and after ExMV-mediated transfer of murine mRNA to human cells, murine-derived proteins were found to be expressed in recipient human cells [14].
Furthermore, the six S. Enteritidis isolates were recovered from different ponds and at different sampling time points; indistinguishable genomic profiles were demonstrated by microarray analysis (see Fig. S1 in the supplemental material).
These microarrays have been demonstrated to be effective for analyzing global gene expression in B. oleracea [ 24].
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