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The results from the microarrays were confirmed by the analysis of 12 genes included in the array by standard MSP and 3 out of 12 genes by pyrosequencing (Figure S3 and S4).
As shown in Figure 3 the changes in gene expression detected with microarrays were confirmed using qRT-PCR for SAP102, HPRT, Fundc2, and BB315069 (3' to Fundc2) in the SAP102 mutants and PSD-95, Fbxo39 and BG092359 (3' to Fbxo39) in the PSD-95 mutants.
The results of microarrays were confirmed using real-time PCR.
Data generated from microarrays were confirmed using real-time PCR (qPCR).
Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR.
In the FO-8 group, gene expression results by microarrays were confirmed by qPCR (88%).
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The down-regulation of NTCP expression observed in microarrays was confirmed at the activity level.
The absence of genes as established with microarrays, was confirmed by PCR.
As shown in Figure 2B, the clec-67 upregulation by S. enterica observed in our microarrays was confirmed by qRT-PCR.
The miRNA expression observed by microarrays was confirmed by qRT-PCR (see Supplementary Figure S4).
Once the preprocessing step is complete, any differences between the microarrays are confirmed to be due to differential expression rather than printing, hybridization, or scanning artefacts.
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microarrays were analyzed
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