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In total 20 microarrays were analysed.
2 microarrays were analysed per strain.
Microarrays were analysed by laser scanning (Hewlett-Packard Gene Scanner).
Two independent cohorts of breast cancer specimens arranged on two different tissue microarrays were analysed for SERBP1 expression by immunohistochemistry.
The genome-wide gene expression profiles determined on Affymetrix microarrays were analysed to detect potential cis-acting elements which may underlie the effects of alcohol.
A selection of 12 genes that showed stable expression across the microarrays were analysed further by QRT-PCR (Genes 1-12; Table 2).
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MLH1 expression on tissue microarrays was analysed as part of a previous study (Cooper et al, 2008).
Nine gill samples from each condition and sampling point, including the five replicates analysed by microarray, were analysed by qPCR.
The expression profiles of 8 genes, belonging to various functional categories and showing various expression patterns in the microarray, were analysed by qRT-PCR under the same conditions as those of the microarray experiment (Additional file 7).
The resulting visualized microarray is analysed on a computer guided reader with automated reading software.
The microarrays data were analysed using BRB ArrayTools (version 4.2).
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