Scanning of the microarrays was achieved by using the Axon Scanner 4000A (Molecular Devices Corp.; Sunnyvale, CA) to obtain 16 bit TIFF image files.
Gene expression profiling on high density DNA oligonucleotide microarrays showed that both qualitative and quantitative improvements were achieved, compared to results with standard muscle homogenate.
"These objectives were achieved.
To investigate the expression profiles under various biotic and abiotic stresses, microarray/MPSS data were achieved from various databases and statistic analyses were carried out (see Methods) to determine if the expression of a lectin gene is regulated by biotic/abiotic stresses.
The read out of microarrays can be achieved using a variety of detection techniques [ 3], however, fluorescence is the most commonly used transduction method [ 4].
The best concordance between qPCR and microarray data is achieved when the observed microarray fold change is between 2 and 7 [ 36], and in the present experiments the genes analyzed by qPCR which had fold changes closest to the lower limit had lower correlations with the microarray data.
The planar microarray format was achieved by an array of microholes fabricated in PDMS.
Validation of the microarray data was achieved using a custom Taqman Low Density Array card (Applied Biosystems).
Validation of microarray data was achieved using cDNA synthesized from eschar specimens A/B, C and E and from R. conorii grown in Vero cells used as reference.
