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In addition, up-regulation on microarrays was validated by independent qPCR on arbitrarily selected genes.
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The expression profiles obtained using custom Agilent microarrays were validated by quantitative RT-PCR.
Taken together, these results show that most of the genes identified by microarrays were validated and only few genes were found to be false positive results.
The microarrays were validated by qRT-PCR assay.
The results obtained with the microarrays were validated for all genes tested.
Sixteen additional genes, over-expressed on microarrays were validated using qRT-PCR.
Microarrays were validated by qRT-PCR analysis of selected transcripts using three housekeeping genes as normalization controls.
Eleven out of 12 genes (6 in SHR, 6 in ACTH) which showed significant variation from WKY in the microarrays were validated by quantitative PCR (Table 3).
* compared with HeLa cells Changes in gene expression detected in the microarrays were validated by Western blotting and real-time PCR.
The expression patterns observed with the microarrays were validated for some relevant genes by real-time quantitative PCR (Table 3[ 7, 24- 26, 28, 29]).
The specificity of the actinobacterial microarray was validated by a set of control hybridizations with 16S rRNA genes clones.
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