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Because the number of pQTL candidates represented in the microarrays was not available, we used the total number of genes under QTL intervals as an approximation.
Of note, the expression of genes selected for methylation-based classifiers assessed on transcript level using microarrays was not significantly altered (Supplementary Table S1).
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Working at a microarray core facility is not where many of us thought we would be today, simply because microarrays were not as popular (or even around!) when we graduated. .
These findings indicate that the differences in regulation by drugs in the microarrays were not due to the selection of the 6 hour time-point.
However, though we detected many alternative splicing events using the whole exon chip, we are aware that these microarrays are not be able to detect exhaustively all alternative splicing in a given tissue.
This issue is difficult to address experimentally because the regulatory elements in the genes that are differentially regulated by E2 and ERβ-selective agonists as observed with the microarrays are not known.
Also rabbit microarrays are not available.
Nevertheless available microarrays are not completely reliable for this purpose.
Genome sequence and microarrays are not yet available for willows.
The branches of pathways containing genes that are not expressed on microarrays were not considered functional.
Alternative explanations such as natural variance in the microarrays are not mentioned.
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CEO of Professional Science Editing for Scientists @ prosciediting.com