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RNA extracted from the hypothalamus microarrays was hybridized with labeled cRNA as described above.
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For mRNA expression experiments, microarrays were hybridized using the MAUI hybridization system (BioMicro), which promotes active mixing during hybridization.
DNA microarrays were hybridized in an ArrayBooster hybridization station (Sunergia Group) for 14 h at 42 °C.
Microarrays were hybridized in Agilent microarray hybridization chambers for 17 h at 60 °C with mixing on an Agilent rotator in a Robbin's Scientific hybridization oven.
Microarrays were hybridized using the two color hybridization method with total RNA extracted from ILTV infected chicken embryo lung cells at 0, 1, 3, 5, and 7 days post infection (dpi).
The microarrays were hybridized and washed in a NimbleGen Hybridization System 4 (NimbleGen Systems, Inc., Madison, WI).
Microarrays were hybridized for 20 hours at 42°C with the NimbleGen Hybridization System 4 (Roche Nimblegen).
Microarrays were hybridized at 65°C for 17 hours in a rotating hybridization oven.
Microarrays were hybridized for 16 hours, and the full protocol for the hybridization can be viewed in Additional File 3, Table S3.
Microarrays were hybridized in static conditions for 16 h in 42 °C in a hybridization chamber (Corning), with the Pronto Universal Hybridization Quick kit.
All microarrays were hybridized together.
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