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Gene expression between the two parental strains (2 × 5 microarrays) was compared using t-tests.
The gene expression of amplified total RNA from melanoma cell lines hybridized to 2000 gene microarrays was compared to that of unamplified total or poly(A)+ RNA using cluster analysis and determining the number of outlier genes between single experiments.
When the intra-platform reproducibility of microarrays was compared to that of TaqMan Gene Expression Assay based real-time PCR, using the 1375 common genes with data in the three platforms, the real-time PCR data shows significantly lower in CV across the whole dynamic range compared to the two microarray platforms.
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P. yoelii genes that did not have probes on both microarrays being compared were excluded.
Genes expressed at Pdetection<0.05 in all never smokers with good quality microarrays were compared to a background of all genes represented by probe sets on the U133A microarray.
In this study, expression measurements for 10,763 genes uniquely represented on Affymetrix U133A/B GeneChips® and Amersham CodeLink™ UniSet Human 20 K microarrays were compared.
When the performance of qPCR-array and microarrays were compared using different aliquots of the same RNA, a low correlation between the two methods (r = -0.443) indicated considerable variability between the two assay platforms.
In order to determine the performance of some of the existing microarrays, Affymetrix Porcine, Affymetrix Human U133+2.0, and the U.S. Pig Genome Coordination Program spotted glass oligonucleotide microarrays were compared for their reproducibility, coverage, platform independent and dependent sensitivity using fibroblast cell lines derived from control and parthenogenic porcine embryos.
The real sensitivity of the microarray was compared for different kinds of fluorescently labelled targets: (a) cDNA and PCR amplified targets, (b) PCR amplified targets labelled using three different labelling methods.
Variance in the distribution of gene expression levels from each microarray was compared between groups of children.
The expression pattern of a number of genes likely to be related to fruit quality was validated by quantitative real-time PCR, and the data from papaya cross-species microarray was compared to microarray data from tomato (a climacteric fruit) and grape (a non-climacteric fruit).
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