Your English writing platform
Free sign upSuggestions(1)
Exact(2)
Unlike most other currently available models [7], [12], [13], GLAM is applicable to microarrays that don't have multiple probes per probeset, and as a result, we are able to test its performance across multiple array platforms.
In our study, Dicer mRNA expression was investigated by RT-qPCR using primers that specifically amplify the three full-length variants and not by transcriptomic microarrays that do not discriminate between these diverse forms of Dicer.
Similar(58)
Quality Control Report was carried out to discard the microarrays that did not fulfill good quality criteria.
During an initial screening, microarrays that did not contain at least 50% of the total number of spots with median background-subtracted signal intensities >3 SD above background in both Cy3 and Cy5 channels were repeated.
However, we only found 1 gene differentially expressed by exon microarray that did not contain the NlaIII restriction site.
The primary aim of this investigation was to examine target labelling methodologies that could be used for our custom miRNA microarray that did not require labour intensive steps such as gel purification, were sensitive and specific, useable at high-throughput and more affordable than multiplex RT-PCR assay.
The individual studies correspond to eight independent microarray datasets that do not share samples or microarrays, and include brain gene-expression measurements from one-day-old and forager honey bees.
Therefore, based on the results of this study, a healthy degree of skepticism is advised when interpreting published results of microarray experiments that do not include validation by technical replication or, preferably, by another technique such as qPCR.
The approach should thus be used with caution and as a complement to classical microarray normalization methods that do not involve correction for histone density, which are also used in this study.
Both RT-qPCR and microarrays are targeted technologies that do not allow the detection of novel miRNAs that are constantly identified [ 97, 98].
Microarray transcripts that did not fully correspond with the RNAseq are annotated as containing probesets that potentially cross hybridize with other closely related genes.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com