Sentence examples for microarrays printed on from inspiring English sources
RNA from immunopurification experiments was hybridized to microarrays printed on aminosilane-coated glass (Schott Nexterion A).
mRNA expression experiments and miRNA experiments were performed with microarrays printed on epoxysilane-coated glass (Schott Nexterion E).
The washing station contains eight relatively large zones (each ∼80 mm2) for multi-stringency posthybridization washing of microarrays printed on glass microscope slides.
RNA from IP experiments was hybridized to microarrays printed on epoxysilane glass (Hendrickson et al., 2009).
In these cases, the measured and fitted results were very consistent across replicate microarrays printed on the same slide.
kisutch), cDNA microarrays printed on slides or nylon membranes, as well as reciprocal suppression subtractive hybridization (SSH), were used to investigate changes in the ovarian transcriptome during primary growth and maturation and bacterial lipopolysaccharide-induced ovarian apoptosis [ 13- 17].
This chapter specifically addresses the effects of scan parameters and limitations of the scanning technology divided into two categories: instrumentation effects (those that arise from the scanning instrumentation itself) and user‐controller parameters (those that an operator chooses) for the most common microarray platform the two‐color cDNA microarray printed on a glass substrate.
The labels were re-suspended in 40 μl of hybridization buffer (40% deionised formamide; 5× Denhart's; 5× SSC; 1 mM Na pyrophosphate; 50 mM Tris pH 7.4; 0.1% SDS) and hybridized onto an RNG-MRC mouse set 25K microarray printed on GE Codelink slide (http://www.har.mrc.ac.uk/services/MPC/microarray/), overnight at 48 °C in a water bath using the Corning hybridization chambers.
Hu and co-workers [ 27] compared amplified and unamplified samples using total RNA obtained from human glioma cell lines and 2300 clone microarrays (printed with duplicates on the same array) to evaluate a similar T7 based protocol with a TS mechanism adopted from Wang and Miller et al. [ 24].
The PCR-amplified cDNAs were resuspended in 50% DMSO (Nacalai Tesque, Kyoto, Japan), and microarrays were printed on UltraGAPS slides (Corning) using an OmniGrid arrayer (Genomic Solutions) with Microarray Stealth Spotting pins (SMP3B, TeleChem).
Microarrays were printed on glass phenyl isothiocyanate slides using Odyssey Calligrapher (BioRad) spotter with a 360 µm pin.
