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The use of plain glass slides, agarose, and a manual arrayer makes this technique useful for developing specialized and inexpensive DNA microarrays on a solid rigid substrate.
We have previously identified selectivity of RIP140 in the regulation of RA target genes by employing microarrays on a genome-wide scale at a 24-hour time point [12].
Agilent microRNA assays integrate eight individual microarrays on a single glass slide.
Agilent miRNA assays integrate eight individual microarrays on a single glass slide.
In addition, multiplex formats were developed that carry several microarrays on a single slide, allowing for cost-effective transcription profiling.
We have validated these infection-specific expression differences by using microarrays on a larger set of samples, and by quantifying the expression of individual genes using RT-PCR.
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We tested the applicability of bulk segregant analysis using the microarray on a sample composed of individuals with known genotypes.
The results demonstrated that the disease-specific transcriptome-based microarray was able to out-perform the generic genomic-based microarray on a number of levels including detection of transcripts and pathway analysis.
Investigations for this patient included a normal echocardiogram, normal sweat chloride, normal chromosomal breakage studies with mitomycin C and DEB and a microarray on a 180 k oligonucleotide platform which did not reveal any microdeletions/microduplications.
To further investigate the association between expression of ABCA1 and survival of ovarian cancer patients, we performed tissue microarray on a different cohort containing 55 ovarian patient samples (Table 4, Figure 5A).
Microarrays scanned on a Genepix 4000A® microarray scanner were analyzed using the Genepix software (Molecular Devices, Sunnyvale, CA).
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