Exact(5)
The decreased intensity observed for non-hemoglobin genes on the post-globin reduction microarrays might be due to high abundance hemoglobin providing a non-specific pseudo-binding signal.
Such microarrays might be a practical solution for the metatranscriptomic study of multi-samples.
Another limitation of microarrays might be the lack of pathway knowledge.
Previously reported evidence suggested that 4q35.2 genes FRG1, 4q35.1 gene ANT1 (SLC25and, and the 5q31 gene PITX1, which were represented on our microarrays, might be involved in FSHD [ 6, 17- 22].
For example, certain data types (e.g. only microarrays) might be entered by a specific centre for another centre or partner, because a certain platform (e.g. Affymetrix) might be available at a certain location or country only (the project started in 2004).
Similar(55)
Overall, the results indicated that the actinobacterial microarray might be widely applicable to the monitoring of changes in planktonic actinobacterial communities in aquaculture environments.
This indicates that the microarray might be useful for analyses of residual ACPA reactivities that are not covered by the conventional ACPA tests like anti-CCP2.
Alternatively, the expression of miR160 and miR390 (and perhaps miR393 which was not represented in the microarray) might be regulated at time points of elicitor treatment not assayed in this work.
Although some LF82-specific genes that were not present on our microarray might be prevalent among IBD isolates, our results suggest that it may be very difficult to identify IBD-specific genetic markers.
However, when a differential diagnosis is needed for an unknown aetiology, the PathogenID v2.0 microarray might be competitive because it does not require (i) designing specific primers for all potential etiologic agents, (ii) setting up the corresponding PCR assays, and (iii) performing the sequence and bioinformatic analyses.
The data processing includes a normalization method to reduce the obscuring variation between microarrays, which might be introduced during the processes of sample preparation, manufacturing, fluorescence labeling, hybridization, and/or scanning.
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