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Three series of eight microarrays from A to H were obtained from the three independent polysomal profile determinations.
Microarrays (from A to H) were exposed to a phospho-imager screen for eight days and scanned with a phospho-fluoroimager (Storm 860, Molecular Dynamics®).
Specifically, we used human microarray data from the Novartis Gene Expression Atlas (http://biogps.gnf.org/) and several microarrays from a human-chimpanzee study [14].
Considering all these problems and knowing the interest of having a reliable normal human gene coexpression network, we have undertaken this task selecting human genome-wide expression microarrays from a controlled set of different normal tissues to build a confident human transcriptomic network using several statistical and computational methods.
By an experiment, we mean a set of microarrays from a particular paper.
The real data consisted of 48 microarrays from a disease challenge experiment in dairy cattle, while the simulated data consisted of 10 microarrays from a direct comparison of two treatments (dye-balanced).
Similar(40)
Using in situ hybridization on tissue microarrays from an independent set of patients, they confirmed significantly differential expression of Spry2 in HCC compared with normal or cirrhotic liver tissue.
Samples were hybridized in 500 μl of 2× SSC (Gibco), 0.1% SDS (Gibco), 5 μg salmon sperm DNA (Invitrogen) on human 25K 50–52mer oligo-microarrays from a national genomic platform (Resogen Program, RNG/MCR, Evry).
Immunohistochemical stain for TAP was applied to a tissue microarray from a breast cancer cohort consisting of 271 patients with a median follow-up time of 5.2 years.
cDNA clones were amplified to generate PCR products for the cDNA microarray from a sequence verified clone collection (Research Genetics, Invitrogen, Huntsville, AL, USA).
Protein expression levels of the various cancer-related gene products were then analyzed in a tissue microarray from a randomized trial of premenopausal breast cancer patients receiving 2 years of adjuvant tamoxifen treatment or no adjuvant treatment.
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