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However, microarrays do not perform well on formalin fixed paraffin embedded tissue (FFPET).
That Affymetrix microarrays do not effectively discriminate between different but closely related duplicated genes has been suggested for allopolyploid wheat [39].
Since Microcebus murinus microarrays do not exist, we decided to use Affymetrix human genome chips since recent studies have illustrated the feasibility of detecting non-human primate brain transcripts using human genome chips [15] [18].
Microarrays do not measure gene expression in absolute units.
In addition, microarrays do not cover all genes in the genome [ 46].
Also, expression microarrays do not provide information regarding alternative splicing and novel transcripts.
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Because satellite repeats weren't considered important, microarrays don't test for their expression, Haber says.
In addition, some tissue samples on tissue microarrays did not adhere to the slide during staining.
However, gene expression was still measured in 1 day intervals, and microarrays did not include all the mouse genes.
Of note, TNF-α expression values obtained by microarrays did not correlate with qPCR performed on un-amplified and un-labelled RNA (not shown).
Because of the complex regulation, coexpression analysis based upon microarrays does not link MITF to its known target genes in different cell types.
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