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The valuable conclusion from their work is that mRNA detection and quantification through high-throughput quantitative PCR or microarrays could result in the first molecular diagnostics for gene expression profile of MII oocytes.
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It has been demonstrated that gene coexpression analysis using too many microarray samples could result in the loss of information.
Given the way microarray data are normalized, this could result in an apparent increase in expression of genes in other tissues, like the eye.
These data provide further evidence of solution phase hybridization behavior in the Codelink microarray system and demonstrate that short hybridization times could result in decreased specificity.
The application of this finding to hybridization protocols could result in a significant improvement of microarray experiments, not limited to expression profiling.
This lack of concordance with our study is surprising, but could result from differences in experimental procedures, microarray platform and chemistry, and statistical analysis.
This could result in the observed variation between qPCR and microarray data.
But lawsuits could result.
Serious hyperglycemia could result.
Injury could result.
This could result in distortion.
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