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Reverse-phase protein microarrays could be a very useful tool in this direction [18].
Although the accuracy of preprocessing algorithms for Affymetrix microarrays could be compared in numerous ways, we have used two performance metrics.
We hypothesized that gene expression profiles from microarrays could be used in combination with mapping data and whole genome sequence information to rapidly identify Avr genes from P. sojae.
In principle, the low specific activity of the IgM mAb for the β1,3 glucan, as assessed with laminarin or oligosaccharides used in ELISA and microarrays, could be even lower for the β1,3 glucan sequences of the secreted native proteins.
Using information downloaded from the National Center for Biotechnology Information (NCBI) website (http://www.ncbi.nlm.nih.gov/Ftp/) on August 8, 2005, each cDNA clone spotted on the microarrays could be associated with the human gene or genomic locus from which it derives [8].
Protein microarrays could be exploited in the screening [ 42].
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Hence, this microarray could be a valuable functional genomics tool to generate reliable expression data from P. vivax field isolates.
Some of the significantly regulated changes found on the microarray could be replicated by quantitative RT-PCR.
With the continuing advancement of protein microarray technology, this microarray could be expanded to hundreds or even thousands of allergens, providing an efficient and economically feasible assay to measure allergen-specific IgE in sera.
To characterize the viral miRNAs, an miRNA microarray could be used in the further studies.
343 of the miRNAs measured by microarray could be detected through small RNA-seq (see Additional file 6).
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