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These differences may be attributable to the fact that microarrays can become saturated with abundantly expressed transcripts, making fold change comparisons unreliable.
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Protocols that require multiple rounds of amplification can accumulate material and labor costs that quickly exceed those associated with the actual microarray analysis, and amplification can become a "rate-limiting" (or "cost-limiting") factor in study design.
While these have been applied in other domains, in the analysis of microarray data, model selection can become difficult since there is often no a priori way of knowing what the structure of the underlying 'true' model might be.
We characterized the gene expression profiles of undifferentiated and dopaminergic lineage-committed hESC-derived cells using a highly focused custom microarray platform (NeuroStem Chip) that can become an important research tool in human stem cell biology.
The use of high-density microarrays is a useful approach to estimate correlations among genes, which in turn can become the basis of transcriptional networks.
Facts can become blurred.
They, too, can become depressed.
This can become a problem.
Retirement can become boring quickly.
So, it can become complicated.
Such polyps can become malignant.
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