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Microarrays at the University Health Network.
The software is designed to evaluate microarrays at the level of gene sets.
RNA was then processed for hybridization on mouse Genome 430 2.0 Affymetrix microarrays at the Erasmus Center for Biomics, using standard Affymetrix protocols.
Of these, five P6 and five P30 samples that exhibited the best quality control parameters on Affymetrix Test3 arrays were then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 microarrays at the Harvard Biopolymers Facility using standard Affymetrix protocols.
Furthermore, they are neither as high-throughput as expression analyses nor as time-efficient to run, and the set-up costs to run mass spectrometry experiments are far higher than microarrays at the present time.
Protein microarrays provide means of rapidly validating the genes identified by expression profiling using DNA microarrays, at the protein level.
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Since the HERC5 gene was not available on the microarray at that time, the IFN-score was based on seven IRGs.
CGH BAC microarrays produced at the Microarray facility of the VU Medical Center were used.
Each sample was hybridized with an Affymetrix Human Genome U133APlus2.0 microarray at the IRB Barcelona Functional Genomics Facility.
Thus indeed, the QPCR results agree with the model deduced using tiling microarray at the stringency level of ≥−1.0.
As a microarray platform for the probes, we applied TubeArray that is a microreaction vial containing a microarray at the bottom.
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