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The Mt16KPlus microarrays are described at http://www.ebi.ac.uk/arrayexpress (accession number A-MEXP-138).
Details of processes for patient accrual, collection of clinical follow up information, pathological review, isolation of nucleic acids, and preparation of tissue microarrays are described previously [5].
Microarrays are described in the Materials and Methods section of text.
The C. glabrata microarrays are described in the Gene Expression Omnibus database ([ 61]; accession number: GPL3922).
Full details of the experiment and preprocessing of the microarrays are described in more detail elsewhere [ 8, 12].
Derivation and implementation of k in correcting pooled DNA estimates of allele frequency using microarrays are described in detail elsewhere [ 26].
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In this work an automated electrical readout system for low-cost glass-slide microarrays is described.
It has been 10 years since tissue microarrays were described by Kononen et al. for the first time.
Generation of PCR amplicons and fabrication of DNA microarrays were described previously [ 88].
For Protocol A, sampling of the chemostat cultures, probe preparation and hybridization to the single-channel Affymetrix GeneChip YG S98 microarrays is described in Piper et al. [ 29].
However, reproducible results on microarrays were described with application of additional rounds of amplification on low amounts of RNA obtained after microdissection [ 6, 9, 10].
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