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Oligonucleotide library management, printing of microarrays and quality control was performed by the Genome BC Microarray Platform (Vancouver, BC, Canada).
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OA and PM produced the home made equine microarrays and check quality.
RJ was involved in overseeing microarray preparation and quality control.
AK carried on the microarray hybridizations and quality control.
Microarray fabrication and quality control have previously been described in von Schalburg et al. [ 30].
All microarray images and quality control measurements were within recommended limits.
Protein microarray printing and quality control protocols have been previously described.
We are greatly indebted to Anne Haegert and Dr. Stéphane Le Bihan at The Jack Bell Prostate Centre, Vancouver, for microarray printing and quality control.
Complete details of cDNA microarray fabrication and quality control are described elsewhere (S. Ralph and co-workers, Gene Expression Omnibus database GEO: GPL5423 and http://www.treenomix.ca/).
Total RNA isolation using a Trizol® method (Invitrogen 155596 018), microarray hybridization and quality checked by Agilent 2100 Bioanalyzer were executed at DNA-Vision.
Microarray spot intensity and quality data were extracted from the scanned images using the BlueFuse software version II (BlueGnome).
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