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Cells at passage 2 7 were used for microarrays and functional studies.
Moreover, the transcriptional profiling of inflammatory genes in peripheral blood mononuclear cells (PBMCs) was explored using microarrays and functional gene expression analysis.
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Gene expression was assessed by microarray and functional module analysis was performed.
In contrast, the CD1c+ PB DC subset was shown by microarray and functional analysis to be an equivalent human population to the murine CD103-CD11b+ myeloid (possibly monocyte-derived) DCs [ 3].
LVL designed microarrays studies and functional experiments and carried out microarrays studies.
We analyzed the immunoregulatory potential of GM-CSF on human monocytes in vitro using microarray technology and functional assays taking all potential functions of human GM-CSF-activated monocytes into account (including gene expression, innate immune functions, interplay with adaptive immunity, wound healing).
Whole genome scanning approaches (microarrays and other functional approaches) are being used in combination with informatics to develop novel lipid-linked targets for drug development.
The lower limits of total RNA required for each method were defined as the lowest RNA input amount where amplification reactions consistently yielded sufficient product to permit analysis on cDNA microarrays and confirmatory functional assays.
The iGBA method requires DNA microarray datasets and functional categories from annotation sources to infer putative gene functions.
In particular, microarray analysis and functional testing revealed CSF-1 as the major chemoattractant for RAW 264.7 macrophages.
Our study illustrates how a combination of microarray technology and functional protein network analysis can be used to identify disease-related key miRNAs.
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