Afterward, 100 random sera from the outpatients were analyzed with our microarrays and the commercial ELISA kits, respectively.
These encompass protocols for the application of homemade microarrays and custom‐designed commercial inkjet microarrays and are tailored for the large‐scale analysis of processing of mRNA, including alternative splicing, as well as for the analysis of processing and modification of noncoding RNA.
Ninety-eight HCC patients who underwent partial hepatectomy or liver transplantation in the Peking University People's Hospital, and commercial microarrays consisting of 46 HCC patients (Shanghai Outdo Biotech, China) were enrolled in this study for immunohistochemical analysis.
We hybridized identical RNA samples to the commercial microarrays and showed that the performance of Sterolgene is comparable to commercial arrays in terms of detection of changes in cholesterol homeostasis and drug metabolism.
To do this, we generated a protein microarray containing surface, cytoplasmic, and commercial extracts from 22 pollen species, commercial extracts from nine non-pollen allergens, and five recombinant allergenic proteins.
Originally, two cost-effective and high-throughput methods including array comparative genomic hybridization (aCGH) and commercial SNP microarrays are used for CNV screening [ 19, 20].
Several sets of external RNA controls are currently available for microarray research [ 4- 9], but few probes corresponding to the controls are available on commercial microarrays and there is no consistency across platforms.
Although Gene Ontology might be inaccurate or partially accurate in some cases, it is still the most used ontology scheme, especially in annotating commercial microarrays and other high-throughput data.
By analyzing OCT2 expression in collected patient tissues and commercial tissue microarray specimens, we demonstrated OCT2 repression in RCC at both transcription and protein levels.
