Sentence examples for microarrays and 2-channel from inspiring English sources
The data sets were generated using one-channel oligonucleotide microarrays and 2-channel custom cDNA microarrays, respectively.
Median signals and background intensities in the hybridized microarrays were acquired from both Cy3 and Cy5 channels using an Agilent High-Resolution Microarray Scanner (Santa Clara, CA).
From these microarray data, we selected Kv1.4 and Kv4.3 channels for further analyses.
The cells expressed subunits of voltage-gated Na+, K+ and Ca2+ channels by microarray and quantitative PCR analysis (data not shown).
The differentiated neurons expressed subunits for voltage-gated Na+, K+ and Ca2+ channels by microarray analysis (data not shown), indicating that the cells contain ion channels characteristic of neurons.
Microarray data were median-normalized in Cy5 and Cy3 channels.
For dual-channels microarray data, the scanning setting for Cy3 and Cy5 channels were balanced by visual inspection of the external control spots.
The quality and homogeneity of the background corrected and VSN normalized microarray data [ 26] for the Cy5 and Cy3 channels was assessed by the Bioconductor package arrayQualityMetrics [ 15] version 2.4.3 http://bioconductor.org/packages/2.5/bioc/html/arrayQualityMetrics.html under R version 2.10.1 http://www.r-project.org/.org/
More genes have low variances in both Cy3 and Cy5 channels and high confidence levels in ADGE microarray than in regular microarray (Fig. 4).
The sample series for each microarray contains the raw data (median signal) of each Alexa637 and Alexa555 channel as well as the normalized data (log2 (Alexa637/Alexa555 ratio)).
Hybridised microarrays were scanned by ScanArray (Perkin Elmer), and spot intensities in Cy3 and Cy5 channels quantified using Bluefuse software (BlueGnome).
