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Pinus radiata transcriptomes were analysed by heterologous hybridisation to the Pinus microarray with cRNA generated from different tissues extracted from ethephon-treated and control plants.
We analyzed the hybridization pattern of this constructed microarray with the Cy3-labeled genomic DNA of various food-borne pathogens and other bacteria.
Simultaneous hybridization of the microarray with two amplified targets labeled with different dyes proved to be efficient, without any competition between the targets.
By means of arrayed primer extension technology, we have designed a genotyping microarray with 204 probe sites for CF transmembrane conductance regulator gene mutation detection.
A microarray with approximately 58,000 probes was used to study dynamic gene expression during kernel development from fertilization to physiological maturity.
The effects of coating the surface of a nucleic acid microarray with polybrene were also studied to assess non-selective adsorption and stability.
A prototype microarray with 117 O and H antigen-specific probes and controls was used to assess probe performance against two pools of gene target PCR amplicons.
A total of 1348 NATs against annotated gene loci have been detected using a custom designed microarray with strand specific probes.
With 10 designed probes, ALV-A, ALV-E, ALV-J, REV, MDV pathogenic and vaccine strains were clearly discriminated on the microarray with the naked eyes.
This 100% correspondence of microarray with qRT-PCR data illustrates that the microarray platform can be used as an effective method for screening large amounts of genes for a particular trait across closely related species.
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Here, we report a novel microarray technology which enables us to explore advanced applications, termed microarray-with-manageable volumes (MMV).
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