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Exact(6)

All signals with MFI score equal or greater than 25% were considered as positive hits, excluding the ones found in the control microarray where the Cy5-Streptavidin alone was probed to determine its non-specific binding to the printed proteins.

Each MRLP fragments carried a unique sequence (complementary Zip-code sequence, cZip-code) which identified a virus by addressed to the location on the microarray where the Zip-code sequence has been spotted.

The electronic nose is a two-dimensional microarray where the individual sections orthogonally vary in their properties on account of two response-modifying strategies (Fig. 8a).

The first array represents each gene of the microarray, where the size is the number of genes.

This involves the substitution of the target DNA from metaphase chromosomes to selected DNA segments spotted onto a microarray, where the distance between target segments determines the resolution.

Additionally, we can quantitatively compare induction between our study and the previously published microarray where the infection protocol and sampling time post-infection where most similar to our study ([ 24]; in which D. melanogaster was infected by septic injury with a mixed bacterial culture and assayed at 12 hours post infection).

Similar(54)

When that is not possible, as for microarrays, where the distribution of fluorescent intensities of individual probes is approximately lognormally distributed, it is common to first take the logarithm of the probe intensity before further statistical analysis [38], [39].

Alternative approaches must be developed for microarrays where the proportion of modulated genes and their distribution are unknown and they may be biased towards up- or down-modulated trends.

It is our view that expression techniques such as microarrays, where the expression of thousands of genes can be monitored simultaneously, can further be used to screen for better predictive genes and develop more accurate diagnostic models.

The mean correlation co-efficient was also determined for the three microarrays hybridised with Cy3-labelled cDNA from ConA stimulated PBLs and Cy5-labelled cDNA from unstimulated PBLs, the average correlation was also determined for the microarrays where the samples were labelled with opposite dyes.

Previously, Shi and Blaschek reported the transcriptional analysis of C. beijerinckii using a ca. 500-gene set DNA microarray where only the expression of genes orthologous to those previously found to be important to the physiology of C. acetobutylicum 824 was examined [ 10].

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