Sentence examples for microarray were hybridized from inspiring English sources
Microarray were hybridized with 33P-labelled probes, first with an oligonucleotide sequence common to all spotted PCR products (5'TcalledGGAAACAGCTATGAC3') called vector probe to measure the amount of PCR products spotted onto the microarray.
The microarray were hybridized in 6 × SSPE/30% formamide at 25°C for 18 h, washed in 0.75 × TNT (Tris-HCl/sodium chloride/Tween) at 37°C for 40 min, and processed by using direct detection of the biotin-containing transcripts by Streptavidin - Alexa647 conjugate.
This microarray was hybridized with cyanine-dye labelled cRNA probes synthesized from RNA from female or male adults of H. contortus.
Depending on the experiment, each microarray was hybridized with one labeled aRNA sample or two samples labelled with different dyes.
For each RNA sample, a single microarray was hybridized with 750 ng Cy3 labeled cDNA using Agilent's standard conditions for single-color microarrays at the Whitehead Center for Microarray Technology.
To this end the microarray was hybridized with RNA extracted from animals that had been treated under different regimes of both cold and salt-induced dehydration.
A direct design was used for hybridization of the two-color arrays (AGL, LGTC), i.e. each microarray was hybridized with two RNA samples from different groups.
The solutions were then denatured at 95°C, and incubated at 37°C for 30 min. The microarray was hybridized overnight using a SureHyb chamber at 65°C for 24 h.
A total of 231,399 oligos for the 27,381 contigs were designed and a 244 K Agilent microarray was hybridized with labeled complex total RNA mixture and genomic DNA.
To assess the ubiquity and specificity of the oligonucleotide probes, the DNA microarray was hybridized in triplicate with genomic DNA isolated from either L. infantum or L. major.
Each microarray was hybridized with 20 μg of fragmented cRNA followed by incubation for 16 h at 45˚C with eukaryotic hybridization controls and herring sperm DNA.
