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Report files summarising the quality of target and control detection for each microarray were generated by GeneChip Operating Software Version 1.4 (GCOS) using the MAS5.0 algorithm (Affymetrix, Santa Clara, CA, USA).
All cDNA clones spotted on the microarray were generated by PCR using GF200 primer pairs, therefore all clones contains 110 bps vector sequence on their GF200 forward primer side.
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The Staphylococcal protein microarray was generated by spotting purified recombinant proteins (0.5 mg/ml in a final glycerol concentration of 40%) in 16 replicates on nitrocellulose coated slides (FAST slides; Whatman) using the ink-jet spotter Marathon (Arrayjet) resulting in spots of ~110 μm in diameter.
For immunohistochemistry, a tissue microarray was generated that contained the following tissues: classic PTC (n = 20), follicular variant of PTC (n = 9), normal thyroid (n = 19), Hashimoto thyroiditis (n = 11), follicular adenoma (n = 15), and follicular carcinoma (n = 14).
The paraffin-embedded cell line microarray was generated as previously described [34].
The human HNSCC tissue microarray was generated and stained for OPN and IKK-β as previously described [31].
Tissue microarray was generated from 38 cHL archival cases from Pathology Department of Universidade Federal de Sao Paulo.
After searching probes for the exon array, the microarray was generated according to eArray guidelines.
A cDNA microarray was generated using 6,912 M. arctica clones printed in duplicate.
Each microarray was generated from RNA harvested from parasites on different days and thus represent independent biological replicates.
A custom RA-MO cDNA microarray was generated using differentially expressed genes obtained from gene subtraction and from comparative whole genome wide U133A analysis in normal donors, active and anti-TNF-α created RA patients.
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