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The expression profiles of candidate genes encoding key transcription factors and enzymes involved in lipid metabolism but not present on the microarray were evaluated by qRT-PCR.
Tissue samples from the immunofluorescence stained tissue microarray were evaluated for the overall intensity of CRHBP related fluorescence detected within the field of view independent from morphological informations of DAPI staining of nuclei.
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The quality of the fluorescent spots on the microarray was evaluated and recorded as present or absent.
The microarray was evaluated for its ability to capture three different strains of influenza A virus, two H1N1, A/Brisbane/59/2007 and A/Solomon Islands/3/2006 and one H3N2, A/Aichi/2/1968.
In this proof of principle study, gene expression microarray was evaluated as a single platform test in the differential diagnosis of common lymphoma subtypes and reactive lymphadenopathy (RL) in lymph node biopsies.
The quality of the microarray was evaluated using the two comparisons: (1) two biological replicates of the same tissue-type labeled with the same dye and (2) the same tissue from two biological replicates labeled with either Cy3 or Cy5 dyes.
Additionally, heterologous hybridization to an R. magnifica microarray was evaluated for two additional symbionts: the symbionts of Calyptogena kilmeri from a Monterey Bay cold seep and the symAVII phylotype of Vesicomya sp. mt-II clams from deep sea vents on the Juan de Fuca Ridge (Goffredi et al., 2003, Stewart et al., 2008).
Besides common laser scanning for fluorescence detection, S-layer based microarrays were evaluated with a compact, low cost platform for direct fluorescence imaging based on surface plasmon enhanced fluorescence excitation.
Statistical samplings of each printed lot of microarrays were evaluated for quality and consistency before use in experiments.
FOG-2 transcript levels determined by microarrays were evaluated for the same 251 tumours.
Five independent and deidentified clinical cohorts of breast cancer tissues, represented as formalin-fixed and paraffin-embedded whole tissue sections or tissue microarrays, were evaluated.
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