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RNA extraction, labeling, and hybridization for microarray were conducted using infested Kavya with GMB4M and uninfested plants as a control.
cDNA labeling, purification and hybridization against the microarray were conducted as previously described [ 25].
Probe synthesis and hybridization to microarray were conducted twice per independent experiment resulting in 12 independent Cy5/Cy3 intensity ratio data points for each spotted cDNA at each time point.
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Microarray was conducted by Nimblegen using a mouse 4-plex expression array (MM8 60mexprxpr ×4).
The microarray was conducted with the Affymetrix HGU133 Plus2 GeneChip Array.
Expression microarray was conducted using samples from 77 ESCC cases (screening group).
Labeling of the RNA and hybridization to the above described high-density microarray was conducted as previously described [ 14].
Oligonucleotide microarray was conducted to investigate gene expression profiles in HL and HCC as outlined in supplementary material Fig. S3.
A microarray was conducted in RasCA-transformed mouse embryonic fibroblasts, showing that many genes encoding cell growth-related proteins are upregulated [ 30].
Independent verification of the expression profiles predicted by microarray was conducted using the same pool of RNA with qRT-PCR and semi-RT-PCR.
To examine the transcription regulation of host cells by nsp11, an RNA microarray was conducted in MARC-nsp11 cells using human gene exon chips.
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