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A double-stranded microarray was washed with PBS 0.01% (v/v) Triton X-100 and blocked with PBS-2PBS-2%v) BSigmaigma) for 1 h.
Subsequently, the microarray was washed with PBS-50 μM zinc acetate-0.5acetate-0.5%n-20 for 10 min, PBS-50 μM zinc aceTween-2010 Triton X-100 for 2 min and PBS-50 μM zinc acetate for 2 min.
The hybridized microarray was washed and then scanned in Cy3 channel with the Agilent DNA Microarray Scanner (model G2565AA).
After hybridization, the microarray was washed 3 times in wash buffer and then labeled for 10 min at 50°C with a solution containing streptavidin-allophycocyanin (1mg/ml final concentration), 6× SSPE, 1× Denhardt's solution, and 0.01% Tween-20.
After removing the cover glass, the microarray was washed twice with 1x SSC, 0.2% (w/v) SDS at 42°C, and then successively with 0.2x SSC, 0.1% (w/v) SDS ,0.2x SSC0.05x SSC and water at room temperature.
After hybridization, each microarray was washed with 2xSSC, 0.1%SDS for 5 minutes at 42°C, followed by 0.1xSSC, 0.1%SDS for 20 minutes at 42°C and rinsed in 5 times in 0.1xSSC at room temperature.
Similar(46)
Hybridized cDNA microarray were washed, dried, and scanned using Scanarray 4000 (GSI Lumonics, Billerica, MA, USA).
Hybridized slides (Human 4x44K Microarray) were washed as recommended and scanned using the High-Resolution Microarry Scanner (Agilent, Santa Clara, CA, USA).
Microarrays were washed, stained, and scanned according to standard procedures.
DNA microarrays were washed as described and read using an Applied Biosystems 1700 Chemiluminescent Microarray Analyzer.
After hybridization, the microarrays were washed with Oligo aCGH wash buffer followed by drying of slides.
More suggestions(15)
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