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A section from the tissue microarray was stained with haematoxylin and eosin to confirm the inclusion of tumor tissue in each core and cores without tumor were excluded from analysis.
The tissue microarray was stained using the Advance Kit (DAKO K5007) according to the manufacturer's instructions.
Tissue microarray was stained using an immunoperoxidase detection technique and scored as described in the Supplementary Materials and Methods.
One section from each microarray was stained with haematoxylin and eosin to confirm the histopathological diagnosis and the adequacy of sampling.
To check the histopathological diagnosis and adequacy of tissue sampling, a section from each microarray was stained with haematoxylin and eosin and examined by an experienced pathologist (TM).
In order to check the histopathological diagnosis and the adequacy of tissue sampling, a section from each microarray was stained with haematoxylin and eosin and examined by light microscopy.
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Microarrays were stained with phycoerythrin-streptavidin (Molecular Probes), scanned with Affymetrix GeneChip scanner and analyzed with Affymetrix Microarray Analysis Suite (MAS) version 5.0.
Once antibody concentration had been optimized with prostate tissue, tissue microarrays were stained.
Microarrays were stained with phycoerythrin-streptavidin (Molecular Probes, Carlsbad, CA), scanned with an Affymetrix GeneChip scanner and analyzed with Affymetrix Microarray Analysis Suite (MAS) version 5.0.
Tissue microarrays were stained with antibodies directed against NURR1 or with normal IgG, and each tissue core was scored according to the intensity of NURR1 staining in epithelial cells (0 = absent and 3 = greatest intensity).
Tissue microarrays were stained for RUNX2 by immunohistochemistry. Anti-RUNX2 antibody validation was confirmed in MDA-MB-231 cells with shRUNX2 knockdown stained using the same protocol as the TMA, and by western blot (supplementary material Fig. S5).
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microarray was deposited
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microarray was confirmed
microarray was rejected
microarray was used
microarray was processed
microarray was designed
microarray was validated
microarray was performed
microarray was generated
microarray was maintained
microarray was assessed
microarray was constructed
microarray was undertaken
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