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Our microarray was spotted in quadruplicate and contained 1990 gene probes that corresponded to ∼98.7% of the coding sequences of this species.
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These microarrays are spotted with 65 base pair oligonucleotides, with each spot representing one of the ∼6700 open reading frames predicted from the analysis of the P. yoelii genomic sequence [41].
Microarrays were spotted at the Laboratory of Molecular Technology, SAIC-NCI Frederick Frederickk, MD) with a BioRobotics Microgrid arrayer (Genomic Solutions, MI).
Membrane blots are generated by fixation of proteins upon growing E. coli clones on membranes and microarrays are spotted using proteins from distinct 1 ml culturing of clones.
The microarrays were spotted at the microarray service (LEFG, CEA, Evry, France) by printing the equine probes, suspended in a spotting buffer composed of 50% dimethyl sulfoxide (DMSO) and TE on hydrogel slides (Schott Nexterion), with a Microgrid-II-robot.
These microarrays were spotted with probes that map to the genes present in a single genome (CGH arrays) or in a number of strains of the same species [ 16, 17], coined pan-genome arrays [ 18].
The microarrays were spotted (LEFG, CEA, Evry, France) by printing the probes (suspended in a spotting buffer composed of 1 1 v/v dimethyl sulfoxide (DMSO) and Tris EDTA buffer) on hydrogel slides (Nexterion, Schott) with a Microgrid-II robot (Genomic Solutions Inc., Ann Arbor, MI).
Locked nucleic acid (LNA -miRNA microarrays were spotted (CastoLNA -miRNA, 2006) using Sanger microarrays2 (Griffiths-Jones et al., 2008) probe sets consisting of 559 human, 170 mouse, and 77 not yet annotated (miRPlus sequences; Exiqon Inc., Vedbaek, Denmark) miRNA probes.
On all microarrays each plasmid solution was spotted in duplicate except the control that was deposited 9 times.
A set of plasmids randomly selected from an E. coli shotgun library of M. ulcerans genomic DNA was spotted on microarray slides.
The equine microarray can be spotted alone on a slide or jointly with the 25 K mouse oligonucleotide microarray [ 23].
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