Two species highly expressed at 3H by the microarray were selected for western blot analysis.
A total of 27 genes with different levels of expression detected using microarray were selected for real-time PCR verification using the same RNA purified for microarray hybridizations.
Candidates identified from the comparison of follicular tissues from the microarray were selected using a multi-level filtering system.
Sequences targeted by the microarray were selected for quantitative PCR analysis to corroborate the results obtained in the microarray (Table 3).
To verify the results of the microarray analyses, 12 differentially expressed genes based on microarray were selected for qRT-PCR to further investigate the expression profiles.
Six different genes found to be regulated in LsRXR RNAi by the microarray were selected and quantified by real time PCR.
In order to confirm microarray data, 11 genes representing a large range of fold change in gene expression as determined by microarray were selected for qRT-PCR analysis (supplementary table S5, Supplementary Material online).
Moreover, the INT-747 samples used for the microarray were selected for a high FXR activation by assessing the mRNA levels of the FXR target gene Shp, to ascertain the identification of FXR targets regulated on the mRNA level.
We also included TarBase version 5.0c [ 73] (June 2008) as a reference database for miRNA target interactions with published evidence; only targets with a "Support Type" value of either "True" or "Microarray" were selected.
According to these criteria, 50 potential marker genes, chosen among those differentially expressed in the microarray, were selected and tested by means of qRT-PCR in leaf, flower (data not shown), seed and mesocarp at five developmental stages in cv Fantasia.
