Sentence examples for microarray was removed from inspiring English sources

After hybridization, the microarray was removed from the MAUI Hybridization Station and immediately immersed in a shallow 250 ml Wash I (Nimblegen, USA) at 42°C for 10 15 s with gentle agitation, then transferred to a second dish of Wash I and incubated for 2 min with gentle agitation.

Low intensity spots of microarray were removed from both channels applying Z-score transformation and we balanced raw intensities applying LOWESS (locally weighted scatter plot smoothing), discarding outlier values from the analysis for every slide.

Two low-quality microarrays were removed from the analysis.

A total of 101 defect arrays and further 801 duplicates of microarrays were removed.

Probe sets declared as present or marginal in less then 80percentt of the analyzed microarrays were removed from the data set.

Two outlier microarrays were removed from each data set (two microarrays from male samples and two from female samples) based on separate hierarchical clustering of each dataset into sample = Cy5 and reference = Cy3 groups.

One sample from the cell line data set was identified as low-quality (based on a very low number of genes detected as present on the microarray) and was removed from further analysis.

For screening of protein microarrays, the GST tag was removed following the final GSH-sepharose washing step by adding thrombin (GE Life Sciences) as suggested by the manufacturer, following by size exclusion filtration using a 50 kDa cut-off Microcon filter (Millipore).

One microarray from the MAS98.06 model was removed due to poor array quality.

A correlation analysis between average absolute fold-changes for all 15 genes showed a low Pearson's correlation coefficient (r = 0.27) probably due to differences in fold-change between the two techniques used, but if c3 (the gene for which fold-change differences between microarray and qPCR were greater) was removed from the correlation analysis, the r value raised to 0.92.

For the microarray experiment stimulations, the medium was removed and 1 ml of new medium (with 0.5% FCS) containing either 10 μl recombinant trout IL-1β protein (rIL-1β) to achieve a concentration of 25 ng/ml or a non-stimulated control with 10 μl PBS.