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The B. henselae protein microarray was probed with a collection of 132 cat sera.
The microarray was probed with sera from the B. mallei infected animals.
A breast cancer progression tissue microarray was probed using anti-MRJ antibody.
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Eleven rSEPs on the microarray were probed with the patient sera.
After a washing step with probing buffer, the microarrays were probed with Cy5-Streptavidin (Invitrogen SA1011) combined with Cy5-goat a-hIgG F ab′ 2 specific (Jackson IR 109-175-097) diluted in probing buffer at 7.5 μg/ml final concentration.
Based on preliminary assay optimization, microarrays were probed with serum diluted 1/150 (IgM detection in monkey sera and IgG in human sera) or 1/500 (IgG in monkey sera).
Epigenome peptide microarrays were probed with mouse serum as described [ 36].
The protein microarrays were probed with 400 predicted and 60 known DNA motifs.
Separate microarrays were probed with independently generated target from each tissue.
C. albicans genomic tiling microarrays were probed pairwise by immunoprecipitated chromatin of a strain producing an HA-tagged protein and a corresponding control strain.
This disparity is mainly due to the differently sized drivers or probes used for hybridization; cDNA subtraction uses kilobase-order biotinylated mRNA as the driver for subtraction, while DNA microarrays are probed by 60-base oligonucleotides.
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