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Each microarray was prepared by robotically spotting peptides, in triplicate, on a glass slide possessing an amino-silane surface coating.
Lectin microarray was prepared as previously described [ 20].
The human genome oligonucleotide microarray was prepared in CapitalBio Corporation (Beijing, China).
The microarray was prepared and the two populations genotyped following the method previously described by Howard et al. [ 73].
The rainbow trout microarray was prepared by spotting of relatively small number of genes (1300) in 6 replicates.
The microarray was prepared by NimbleGen Systems Inc. following the method reported by Saldivar [ 14] (GEO approved http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE20605.nih.gov/geo/query/acc.cgi acc=GSE20605
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RNAs to be used for microarray were prepared as previously described [18].
Labeled probe for hybridizations with the NSF45K microarray were prepared from 1 2 µg mRNA samples using the SuperScript™ indirect cDNA Labeling System (Invitrogen, Carlsbad, CA).
Five independent biological replicates of microarray were prepared for each strain of mice.
Paraffin-embedded sections (5 μm) of a tissue microarray were prepared and labeled with antibodies, in accordance with standard protocols.
The paraffin-embedded ovarian cancer patients' tissue microarray were prepared and retrieved from the Department of Pathology, Tri-Service General Hospital as described previously [ 33].
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