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The rectangular sensor chip containing the mycotoxins microarray was placed into the chip holder with the hemisphere prism, and a microfluidic cell was installed on top.
The target (28 μl) was applied on the cover slip (24 32 mm) and the microarray was placed on top of the cover slip.
The target (60 μl) was applied on the cover slip and the microarray was placed on top of the cover slip.
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The microarrays were placed in a Hybridization Oven 640 (Affymetrix) at 45°C for 16 hours, rotating at 60 RPM.
The microarrays were placed in the ProPlate multi-array system (Grace Bio-Labs).
Microarrays were placed into the Amersham Lucidea hybridization station (Amersham) and hybridized with hybridization mixtures containing 20 μg of labeled cRNA at 37°C for 16 hours.
The probed microarray slide was placed in ArrayIt microarray high-speed centrifuge and the residual WBF buffer removed via a 30 seconds of centrifugation.
The microarray slide was placed probe side towards the target.
Then, one microarray slide was placed onto the gasket slide, with the active side facing down.
First, the microarray slide was placed inside a Gentel SIM plex 16 Multi-Array device bottom holder piece, next the silicone gasket was carefully placed on the top of the slide and the device top piece held in place with tightening screws.
In cDNA microarray analysis, special emphasis was placed on the quality of tissue material.
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