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This microarray was not designed to be diagnostic; by using whole-genome arrays for each virus, probes were designed independent of degree of cross-hybridization.
Since a commercial Phaseolus vulgaris microarray was not available, a soybean (Glycine max L. Merr).
Because the di-peptide nitrogen source microarray was not comprehensive, the amino acid sample size was variable.
The first commercially available whole-transcriptome equine-specific microarray was not published until 2009, therefore early studies attempted two different approaches.
Cross-hybridization between the Cy3-labelled (cZIP on the) padlock probe and other ZIP-codes that were used on the microarray was not observed (results not shown).
Microarray results may reflect expression of microRNA precursors or processed microRNAs, because RNA hybridized to the microarray was not specifically selected to include small RNA species.
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Approximately half of the highly expressed genes identified in the 135k microarray are not included in the previous microarray.
FGF4 and Cacnb3, although displayed on the microarray, were not amplified by qRT-PCR.
However, it is evident that the overall expression alteration of all 25 genes is statistically comparable, although the actual fold change values in real-time PCR and microarray are not always commensurable.
Since most of the miRNAs used on the microarray are not present at detectable levels in most serum samples, clustering was only performed on a subset of the miRNAs.
Since the samples for the microarray were not pooled, we could perform one-way analysis of covariance (ANCOVA) for covariates, age, PMI, MDD status, viral load, were inputted into the model.
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