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The RIN value of the RNA samples before being applied to the microarray was measured using an Agilent 2100 Bioanalyzer (USA) and was 10.0.
The lower limit of signal detection on the Bovine Innate Immune Microarray was measured using 'spike in' RNA transcripts of known quantities from the commercial Lucidea Microarray Scorecard (Amersham Biosciences).
Blood was obtained before and after the challenge, total RNA was extracted from mononuclear cells, and signal intensity of the labeled cDNA hybridized to a 3800-gene oligonucleotide microarray was measured.
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The piezoelectric properties of microarrays are measured by piezoelectric force microscopy (PFM).
In order to validate this finding, the correlation coefficients to the ISG15 gene in the validation data set generated by the custom affymetrix microarray (N=111, EOS Hu03 microarrays) were measured.
The expression of 10 miRNAs that showed differential expression on the microarrays were measured in 12 RNA samples (3 preadipocyte and 3 adipocyte samples from both brown and white fat) using the Taqman® MicroRNA assays (Applied Bioystems) that detect mature miRNA.
Much higher values (for instance a fold change of 91 ± 17 for control B lymphoblasts compared to 12.5 from microarrays) were measured for ASS, demonstrating that for determining high difference levels the microarrays data saturate at lower values than those resulting from quantitative RT-PCR.
Relative expression of the miRNAs identified from microarray analysis was measured by real-time PCR.
The first Saccharomyces cerevisiae cell cycle microarray dataset was measured through the regulatory responses under the elutriation treatment, available at the Stanford Microarray Database.
Since the microarray data was measured by the ratio of the hybridization signal for each gene, it could vary by factors of 2 or greater.
The quality of RNA used for microarray analysis was measured using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), according to the manufacturer's instructions.
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