Sentence examples for microarray was incubated from inspiring English sources

To visualise antibody binding, the microarray was incubated with 3′-diaminobenzide and counterstained with Mayer's Haematoxylin.

The microarray was incubated for 90 minutes at RT in the dark.

A protein microarray was incubated with a mixture of fluorescently labeled in vitro transcribed ASH1 mRNA and poly(A -selected totA -selectedm cells harvestotalt mRNAlog phase in YPD (fromre 1A).

The microarray was incubated overnight at 42°C in a hybridization cassette (Telechem International).

The hybridized microarray was incubated for 16 hrs at 42°C and then scanned with an Affymetrix 428 scanner (Affymetrix, USA).

For the detection the microarray was incubated with streptavidin HRP conjugate (R&D Systems) followed by dye precipitation reaction using TrueBlue™ (KPL, Gaithersburg, MD, USA).

14 As a screen to identify materials with broad spectrum resistance to bacterial attachment, the polymer microarray were incubated with three different green fluorescent protein (GFP -labelled bacterial species, Pseudomonas aeruGFP -labelledStaphylococcus aureus 8325-4 and uropathogenic Escherichia coli O6:K15:H31 (UPEC) for 72 h.

Each one of four microarrays was incubated with 30 µl of one of four different extension solutions (A, C, G or U) containing 1 x extension buffer (26 mM Tris-HCL pH 9.5, 6.5 mM MgCl2), 3.2 µM array-specific Cyanine-labeled dideoxynucleotides (ddNTP) and 6.6 U/µl thermosequenase DNA polymerase (USB Corporation).

Microarrays were incubated overnight (16 20 hours) at 50°C in a dark humidified chamber (Genetix).

For labeling, microarrays were incubated for 30 min with ExtrAvidin Peroxidase (Sigma) diluted 1∶1000 in BSA Peroxidase Stabilizer (BioFX).

Afterwards, the microarrays were incubated for 16 h in a wet chamber to ensure efficient covalent binding of the oligonucleotides to the surface.