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For immunohistochemistry, a tissue microarray was generated that contained the following tissues: classic PTC (n = 20), follicular variant of PTC (n = 9), normal thyroid (n = 19), Hashimoto thyroiditis (n = 11), follicular adenoma (n = 15), and follicular carcinoma (n = 14).
The paraffin-embedded cell line microarray was generated as previously described [34].
The human HNSCC tissue microarray was generated and stained for OPN and IKK-β as previously described [31].
A cDNA microarray was generated using 6,912 M. arctica clones printed in duplicate.
After searching probes for the exon array, the microarray was generated according to eArray guidelines.
Each microarray was generated from RNA harvested from parasites on different days and thus represent independent biological replicates.
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Report files summarising the quality of target and control detection for each microarray were generated by GeneChip Operating Software Version 1.4 (GCOS) using the MAS5.0 algorithm (Affymetrix, Santa Clara, CA, USA).
All cDNA clones spotted on the microarray were generated by PCR using GF200 primer pairs, therefore all clones contains 110 bps vector sequence on their GF200 forward primer side.
First generation MHC microarrays were generated by direct spotting of the pMHC of interest onto the microarray slide.
DAT image and CEL intensity files of the microarrays were generated using the Affymetrix GeneChip® Command Console (version 0.0.0.676, Affymetrix).
We describe a novel, simple and low-cost protein microarray strategy wherein the microarrays are generated by printing expression ready plasmid DNAs onto slides that can be converted into protein arrays on-demand.
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