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One microarray was discarded from the 46 because of lower hybridization signals, confirming the good quality of the technical procedures (see Materials and Methods), and leaving a total of 45 birds for further study.
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Any genes that were not included in our microarrays were discarded and we then used a set which contained 1006 genes.
These 5 microarrays were discarded among a set of 60 microarrays corresponding to a larger experimental design with 6 treatments, confirming a global good quality of the technical procedures.
The supernatant was discarded and the microarrays were blocked by incubation with 100 μl blocking buffer at 300 spm for 5 min.
Visual inspection of the microarray images filtered out florescence most likely not due to labeled cDNA binding; the data from these array elements was discarded.
The glycerol was discarded.
The wash was discarded.
Supernatant was discarded.
The medium was discarded.
One questionnaire was discarded.
The membrane was discarded.
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