Sections (4 μm) of the tissue microarray were dewaxed, rehydrated and an antigen retrieval step performed when required.
Tissue microarrays were dewaxed in xylene and rehydrated through graded alcohol.
Pancreatic tissue microarrays were dewaxed and rehydrated before antigen unmasking, using target retrieval solution (DAKO Corporation, Carpenteria, CA, USA), in a water bath for 30 min.
A TissueFocus Cancer Survey Tissue Microarray (Origene, Rockville, MD, USA) was dewaxed and endogenous peroxidase activity was blocked with 0.5% hydrogen peroxide in methanol.
Tissue microarray sections were dewaxed in xylene and rehydrated, then unmasked in retrieval solution (S2367, DAKO) at 121°C in a pressure cooker for 5 minutes.
Tissue microarray sections were dewaxed in xylene and rehydrated through graded concentrations of ethanol to distilled water.
Tissue microarray slides were dewaxed and rehydrated using xylene and a series of graded alcohols, followed by heat-induced antigen retrieval using a target retrieval solution (S2031, DakoCytomation, Glostrup, Denmark) in a pressure cooker for 10 min.
Tissue microarray (TMA) slides were dewaxed at 55°C for 20 min followed by three 5 min washes with xylene.
Tissue microarray sections (5 μm) were dewaxed with xylene and rehydrated in ethanol before microwave antigen retrieval in target retrieval solution, pH 6.
Tissue microarrays sections (5 μm) were dewaxed with xylene, rehydrated in ethanol before microwave antigen retrieval, and stained for GPER expression using ab12564 and ab12563 (Abcam, Cambridge, UK) and for ER α expression using anti-ER α M7047 (Dako, Copenhagen, Denmark).
