As all probes on the microarray were derived from potato, we first assessed whether the potential sequence divergence of these probes would affect signal intensities.
cDNA sequences for the PtGen2 microarray were derived from thirty-six cDNA libraries constructed primarily from loblolly root, stem and needle tissues [ 32].
The majority of the 13907 probes spotted on the microarray were derived from RJ and WL brain- and testis Expressed Sequence Tag (EST) libraries (n = 12742), but an additional 1136 clones were specifically chosen because of their biological functions.
While most of the genes on the microarray were derived from the head library, interestingly, some genes originating from only the larval midgut library were expressed in the head (Table 1).
The majority of the clones incorporated on the microarray were derived from the BoMP cDNA library generated from bovine myeloid cells subjected to various stimuli, including over 900 sequences unique to the library.
ERs from microarrays are derived from two differently labeled but mixed samples that competitively hybridize to immobilized gene-specific probes.
The cell intensities and detection from the nine hybridized oligonucleotide microarrays were derived by use of the Affymetrix Microarray Suite 5 (MAS 5.0) with default settings.
At intervals of 4 h from Zt0 to Zt20, three mice from each group are sacrificed, and microarrays are derived from pooled RNA samples from the livers of each set of mice.
The 3, 320 xylem unigenes on the microarrays were derived from relatively abundant xylem transcripts in radiata pine [ 42] and they may only represent 30-40% of genes moderately to highly transcribed during wood formation [ 44].
The genes for the microarrays were derived from a series of display (differential display reverse transcriptase-PCR, subtractive hybridization with magnetic beads, and cDNA-AFLP display) experiments that compared the transcriptome of plants attacked by the larvae of its specialist herbivore, Manduca sexta, with that of unattacked plants [ 9- 11].
